PERBEDAAN ANTIKOAGULAN EDTA DAN HEPARIN TERHADAP NILAI HEMATOKRIT

widia rahmatullah, Rahmin B. Labito, Resmi Aini, Rudina Azimata R, Reska Handayani

Abstract


Hematocrit examination is one of the examinations to determine the volume of erythrocytes contained in the blood. Determination of the hematocrit value can be done by the microhematocrit method, using a sample of venous blood mixed with an anticoagulant. Anticoagulant Ethylene Diamine Tetra Acetate (EDTA) can bind calcium ions (Ca) so that clotting does not occur but the use of inappropriate concentrations and volumes of EDTA anticoagulants can cause low hemoglobin levels as well as hematocrit values. Heparin anticoagulants can inhibit the conversion of prothrombin into thrombin, but if used incorrectly it causes cells to agglomerate resulting in invalid calculations. This study aims to determine the average and differences in hematocrit values using EDTA and heparin anticoagulants. This type of research is descriptive analytic which is a research method that functions to describe an object under study. The research analysis used univariate analysis to obtain the average hematocrit value and bivariate analysis used the Independent T-test to determine differences in the results of the hematocrit values using EDTA anticoagulants and heparin anticoagulants. The results of the examination of the hematocrit value of the average EDTA anticoagulant were 38.49% while the average Heparin anticoagulant hematocrit value was 40.05%. Difference in the average value of hematocrit is 1.56%. The results of the Independent T-test statistic where the p value > 0.05 is sig 0.205, which means that the Ho hypothesis is accepted. So it can be concluded that there is no significant difference between the hematocrit values using EDTA and heparin anticoagulants.


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References


Adzaki, M.Z.2018, Pengaruh Volume Darah pada Tabung Vacuntainer K3EDTA Terhadap Nilai LED Metode Westergren. Karya Tulis Ilmiah, D3 Analisis Kesehatan, Universitas Muhammadiyah, Semarang.

Arningsih, W. 2017, Pengaruh Antikoagulan K3EDTA dan Heparin Vakuntainer Terhadap Jumlah Trombosit dengan Metode Automatik, Karya Tulis Ilmiah, D3 Analisis Kesehatan, Universitas Muhammadiyah, Semarang.

Astuti, Y., & Artini, D. 2019, Comparative Hemoglobin and Hematocrit Before and After Donation To Blood Donate in Unit Transfusion Yogyakarta City. Jurnal Riset Kesehatan, 8(2) : 40-42

Departement Kesehatan Republik Indonesia Nomor 9. Tahun 2015. Tentang Standar Pelayanan Transfusi Darah. 31 Desember 2015. Jakarta.

Fitria, L., Illiy, L. L., & Dewi, I. R. 2017. Pengaruh Antikoagulan dan Waktu Penyimpanan terhadap Profil Hematologis Tikus (Rattus norvegicus Berkenhout, 1769) galur wistar. Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal, 33(1) : 22-30.

Gandasoebrata, R. 2013. Penuntun Laboratorium Klinik. Cetakkan Kelimabelas Dian Rakyat, Jakarta.

Kiswari, Rukman. 2014, Hematologi dan Transfusi. Erlangga, Jakarta.

Kumala, I. D., dan Rahayu, S. 2019, Pengetahuan Tentang Donor Darah dan Perilaku Altruisme pada Mahasiswa. Jurnal Kesehatan Cehadum, 1(1) : 59-69.

Mardiana dan Rahayu, Ira Gustira. 2017. Pengantar laboratorium Medik, Jakarta.

Muslim, Azhari. 2017, Pengaruh Waktu Simpan Darah K2EDTA dan Na2EDTA pada Suhu Kamar Terhadap Kadar Hemoglobin. Jurnal Analis Kesehatan. 4 (2) : 392-396.

Notoatmodjo, S. 2018. Metodologi Penelitian Kesehatan. Rineka Cipta, Jakarta

Nugraha, Gilang. 2015,Panduan Pemeriksaan Laboratorium Hematologi Dasar. CV Trans Info Medika, Jakarta.

Oktaviani, M. A., & Notobroto, H. B. 2014. Perbandingan Tingkat Konsistensi Normalitas Distribusi Metode Kolmogorov-Smirnov, Lilliefors, Shapiro-Wilk, dan Skewness-Kurtosis. Jurnal Biometrika dan Kependudukan, 3(2) : 127-135.

Plebani, M. 2012. Quality Indicators to Detect Pre-analytical Errors in Laboratory Testing. The Clinical Biochemist Reviews, 33(3): 85.

Priyana, A. 2021, Perdarahan Iliopsoas pada Pasien Covid-19 dalam TerapiAntikoagulan. Ebers Papyrus, 27(1) : 116-123.

Riswanto . 2013. Pemeriksaan Laboratorium Hematologi, Alfa Medika dan Kanal Medika, Yogyakarta.

Rosidah, R,& Wibowo, C. 2018, Perbedaan antara Pemeriksaan Antikoagulan Edta dan Heparin Terhadap Nilai Hematokrit (HCT). Jurnal Sains, 8(16) : 16-21.

Sayekti, S., & Puspitasari, M. T. 2017, Perbedaan hasil Pemeriksaan Mikro Hematokrit menggunakan EDTA 5% dan EDTA 10% : Studi pada Mahasiswa STIKes ICME Jombang D3 Analis Kesehatan Kelas B Semester VI. Jurnal Insan Cendekia, 4(1).

Stokol, T., Priest, H., Behling-Kelly, E.,and Babcock, G. 2014, Samples for Hematology, Animal Healths Diagnostic Center. Clinical Pathology Laboratory College ofVeterinary Medicine Cornell University, Ithaca, New York.

Subiyono, S., Martsiningsih, M. A., & Gabrela, D. 2016. Gambaran Kadar Glukosa Darah Metode GOD-PAP (Glucose Oxsidase–Peroxidase Aminoantypirin) Sampel Serum dan Plasma EDTA (Ethylen Diamin Terta Acetat). Jurnal Teknologi Laboratorium, 5(1) : 45-48.

Sugiyono. 2015a. Metotologi Penelitian Kuantitatif Kualitatif dan R&D. Alfabeta, Bandung

Susilo, T. D. E., Supadmi, F. R. S., & Artini, D. 2020. Pengukuran Kadar Hematokrit dan Hitung Jumlah Eritrosit pada Komponen Darah Packed Red Cells (PRC) Selama Pengolahan dan Penyimpanan di UTD PMI Kota Yogyakarta. Pin-Litamas, 2(1) : 22-27.

Tika. 2018 ,Perbedaan Penggunaan Antikoagulan EDTA dan Heparin Terhadap Nilai Hematokrit Metode Mikro, Karya Tulis Ilmiah, D3 Analisis Kesehatan, Universitas Muhammadiyah, Semarang.

Tumpuk, S., & Suwandi, E. 2018. Perbedaan Hasil Pemeriksaan Mikro Hematokrit Menggunakan Makrosentrifus dengan Mikrosentrifus. Jurnal Laboratorium Khatulistiwa, 1(2) : 142-144.

Wu, X., Zhao, M., Pan, B., Zhang, J., Peng, M., Wang, L.,& Shang, H. 2015. Complete Blood Count Reference Intervals for Healthy Han Chinese Adults. PloS one, 10(3)




DOI: http://dx.doi.org/10.30633/jsm.v6i1.1870

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